Hotspot

trnP-CCA-psaJ

LSC · 66319-66682 · 364 bp

Back to Malcolmia

Pi 0.00275

MarkerSeek score 55.00

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 1
pi: 0.00275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	ACCCGTGACATTTTGTACCC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	493 bp	1.0	77.4
trnP-CCA-psaJ_p2	CCCGTGACATTTTGTACCCA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	492 bp	1.0	77.4
trnP-CCA-psaJ_p3	ACAGGATTTGAACCCGTGAC	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	504 bp	1.0	77.3
trnP-CCA-psaJ_p4	CAGGATTTGAACCCGTGACA	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	503 bp	1.0	77.3
trnP-CCA-psaJ_p5	TGACAGGATTTGAACCCGTG	AAACCTGCTAACGAACCGAA	50.0	45.0	58.1	57.8	506 bp	1.0	77.3