Hotspot

rbcL-accD

LSC · 56230-56901 · 672 bp

Back to Malcolmia

Pi 0.00149

MarkerSeek score 59.60

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 1
pi: 0.00149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CGAAGCTTGCAAATGGAGTC	CGTTGCTTTACTTAGCTCGC	50.0	50.0	57.7	57.6	850 bp	1.0	74.7
rbcL-accD_p2	GAGTCCTGAACTAGCTGCTG	CGTTGCTTTACTTAGCTCGC	55.0	50.0	57.7	57.6	835 bp	1.0	74.4
rbcL-accD_p3	AAGCTTGCAAATGGAGTCCT	CGTTGCTTTACTTAGCTCGC	45.0	50.0	57.7	57.6	848 bp	1.0	74.3
rbcL-accD_p4	CGAAGCTTGCAAATGGAGTC	GGAGCAAAACTATCCGTTGC	50.0	50.0	57.7	57.5	864 bp	1.0	73.7
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	GGAGCAAAACTATCCGTTGC	55.0	50.0	57.7	57.5	849 bp	1.0	73.4