Hotspot

trnL-UUA-trnF-UUC

LSC · 47217-47814 · 598 bp

Back to Malcolmia

Pi 0.00394

MarkerSeek score 62.90

Primer available yes

Label trnL-UUA-trnF-UUC

Feature Metadata

label_name: trnL-UUA-trnF-UUC
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 2
pi: 0.00394
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UUA-trnF-UUC_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	597-688 bp	1.0	80.0
trnL-UUA-trnF-UUC_p2	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	594-685 bp	1.0	76.8
trnL-UUA-trnF-UUC_p3	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	586-677 bp	1.0	76.1
trnL-UUA-trnF-UUC_p4	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGG	50.0	55.0	57.5	58.6	559-650 bp	1.0	75.7
trnL-UUA-trnF-UUC_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	569-660 bp	1.0	75.6