Hotspot

trnS-AGC-trnG-GGA

LSC · 7802-8452 · 651 bp

Back to Malcolmia

Pi 0.00307

MarkerSeek score 60.50

Primer available yes

Label trnS-AGC-trnG-GGA

Feature Metadata

label_name: trnS-AGC-trnG-GGA
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 2
pi: 0.00307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-AGC-trnG-GGA_p1	ACGCTTTAATCCACTCAGCC	TGAATCAAACCGAGAGACCC	50.0	50.0	58.3	57.2	758-762 bp	1.0	72.0
trnS-AGC-trnG-GGA_p2	CGCTTTAATCCACTCAGCCA	TGAATCAAACCGAGAGACCC	50.0	50.0	58.3	57.2	757-761 bp	1.0	72.0
trnS-AGC-trnG-GGA_p3	ACGCTTTAATCCACTCAGCC	GAATCAAACCGAGAGACCCT	50.0	50.0	58.3	56.9	757-761 bp	1.0	69.6
trnS-AGC-trnG-GGA_p4	CGCTTTAATCCACTCAGCCA	GAATCAAACCGAGAGACCCT	50.0	50.0	58.3	56.9	756-760 bp	1.0	69.6
trnS-AGC-trnG-GGA_p5	ATCCACTCAGCCATCTCTCC	TGAATCAAACCGAGAGACCC	55.0	50.0	58.6	57.2	750-754 bp	1.0	69.5