Hotspot

rpl32-trnL-UAG

SSC · 115509-116463 · 955 bp

Back to Maianthemum

Pi 0.0289

MarkerSeek score 72.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 955
variable_sites: 69
pi: 0.0289
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TACCTAACCCATTGCGGAAC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.6	58.0	1147-1679 bp	1.0	85.5
rpl32-trnL-UAG_p2	TACCTAACCCATTGCGGAAC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.6	58.0	1147-1680 bp	1.0	85.5
rpl32-trnL-UAG_p3	TACCTAACCCATTGCGGAAC	TTTACAGTATGCCGCCACTC	50.0	50.0	57.6	58.0	1147-1739 bp	1.0	85.3
rpl32-trnL-UAG_p4	TACCTAACCCATTGCGGAAC	GACGAATCACGCAACCCTAT	50.0	50.0	57.6	58.1	1146-1930 bp	1.0	84.5
rpl32-trnL-UAG_p5	TACCTAACCCATTGCGGAAC	CGAATCACGCAACCCTATGA	50.0	50.0	57.6	58.1	1147-1928 bp	1.0	84.5