Hotspot

ndhF-rpl32

SSC · 114341-115328 · 988 bp

Back to Maianthemum

Pi 0.0183
MarkerSeek score 67.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
988
variable_sites
52
pi
0.0183
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CACTCGGAAAAGCCCACATA ACTTGGTCTTGAGTTAGGCG 50.0 50.0 57.9 57.5 1612-1661 bp 0.888889 78.6
ndhF-rpl32_p2 CACTCGGAAAAGCCCACATA CTTGGTCTTGAGTTAGGCGT 50.0 50.0 57.9 57.5 1611-1660 bp 0.888889 78.6
ndhF-rpl32_p3 AGCAACTGCACCAACGAATA ACTTGGTCTTGAGTTAGGCG 45.0 50.0 58.1 57.5 2196-2245 bp 0.888889 77.3
ndhF-rpl32_p4 AGCAACTGCACCAACGAATA CTTGGTCTTGAGTTAGGCGT 45.0 50.0 58.1 57.5 2195-2244 bp 0.888889 77.3
ndhF-rpl32_p5 TAGCAACTGCACCAACGAAT CTTGGTCTTGAGTTAGGCGT 45.0 50.0 58.1 57.5 2196-2245 bp 0.888889 77.3