Hotspot

rps3-rpl22

LSC · 85193-85248 · 56 bp

Back to Maianthemum

Pi 0.0625

MarkerSeek score 65.80

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 8
pi: 0.0625
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	GCGTTCGCCCCTCTATTAAT	CGGGCGTTCTTATGAGGAAA	50.0	50.0	57.8	58.0	594-611 bp	1.0	82.6
rps3-rpl22_p2	GCGTTCGCCCCTCTATTAAT	TCTGACTTTCGCGAGACATG	50.0	50.0	57.8	58.0	792-840 bp	1.0	82.3
rps3-rpl22_p3	GCGTTCGCCCCTCTATTAAT	ATGTCTGTTTTCAAAGCGCG	50.0	45.0	57.8	58.0	635-652 bp	1.0	82.2
rps3-rpl22_p4	GCGTTCGCCCCTCTATTAAT	CATTCATTGGCGAGGGAGAA	50.0	50.0	57.8	58.0	717-734 bp	1.0	82.1
rps3-rpl22_p5	GCGTTCGCCCCTCTATTAAT	GGCGAGGGAGAAACCTTATG	50.0	55.0	57.8	58.0	709-726 bp	1.0	82.1