Hotspot

psbE-petL

LSC · 66100-67418 · 1319 bp

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Pi 0.0137

MarkerSeek score 70.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1319
variable_sites: 48
pi: 0.0137
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1423-1467 bp	1.0	81.9
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1436-1480 bp	1.0	81.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCAGCCAATAGAAAACCGA	45.0	45.0	58.2	57.5	1432-1476 bp	1.0	80.9
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.8	57.5	1431-1475 bp	1.0	80.8
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	50.0	45.0	58.2	57.5	1425-1469 bp	1.0	80.4