Hotspot

petA-psbJ

LSC · 64259-65330 · 1072 bp

Back to Maianthemum

Pi 0.014

MarkerSeek score 57.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1072
variable_sites: 42
pi: 0.014
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.1	58.0	1174-1201 bp	1.0	82.7
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	1191-1218 bp	1.0	82.1
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.1	58.1	1175-1202 bp	1.0	82.1
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	1192-1219 bp	1.0	81.4
petA-psbJ_p5	TGTTCTTCTTGGCATCCGTT	CCGGATTGGGTTCATCTCTG	45.0	55.0	57.7	58.0	1184-1211 bp	1.0	81.2