Hotspot

rbcL-accD

LSC · 57902-58597 · 696 bp

Back to Maianthemum

Pi 0.0128

MarkerSeek score 62.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 696
variable_sites: 26
pi: 0.0128
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TCGAACCAGTGGATACGGTA	CCGGTATGTCCAACAGCATT	50.0	50.0	57.9	58.2	814-835 bp	1.0	82.0
rbcL-accD_p2	CGCTGCTTGTGAAGTATGGA	CCGGTATGTCCAACAGCATT	50.0	50.0	58.3	58.2	852-873 bp	1.0	80.6
rbcL-accD_p3	GTTCGAACCAGTGGATACGG	CCGGTATGTCCAACAGCATT	55.0	50.0	58.4	58.2	816-837 bp	1.0	80.2
rbcL-accD_p4	TCGAACCAGTGGATACGGTA	CTTCCACCGGTATGTCCAAC	50.0	55.0	57.9	58.3	823-841 bp	0.555556	62.9
rbcL-accD_p5	CGCTGCTTGTGAAGTATGGA	CTTCCACCGGTATGTCCAAC	50.0	55.0	58.3	58.3	861-879 bp	0.555556	61.6