Hotspot

ndhC-trnV-UAC

LSC · 51077-52740 · 1664 bp

Back to Maianthemum

Pi 0.0106
MarkerSeek score 54.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1664
variable_sites
48
pi
0.0106
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 36.4 55.0 56.1 58.7 1700-1730 bp 1.0 46.4
ndhC-trnV-UAC_p2 TCATATTCGTGAAGCAGAAACA TTCGAGTCCGTATAGCCCTA 36.4 50.0 56.1 57.1 1699-1729 bp 1.0 44.3
ndhC-trnV-UAC_p3 CATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 38.1 55.0 54.6 58.7 1699-1729 bp 1.0 42.9
ndhC-trnV-UAC_p4 ATCATATTCGTGAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.6 58.7 1701-1731 bp 1.0 42.9
ndhC-trnV-UAC_p5 TCATATTCGTGAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 34.8 55.0 56.6 58.7 1700-1730 bp 1.0 42.9