Hotspot

psbM-trnD-GUC

LSC · 30573-31302 · 730 bp

Back to Maianthemum

Pi 0.00902

MarkerSeek score 63.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 730
variable_sites: 20
pi: 0.00902
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TGCTATACACCTTGCTGGGA	45.0	50.0	55.8	58.4	729-873 bp	1.0	64.7
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	703-847 bp	1.0	64.3
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	ACACCTTGCTGGGATTGTAG	45.0	50.0	55.8	57.5	723-867 bp	1.0	64.1
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	CACCTTGCTGGGATTGTAGT	45.0	50.0	55.8	57.5	722-866 bp	1.0	64.1
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	ATGCTATACACCTTGCTGGG	45.0	50.0	55.8	57.4	730-874 bp	1.0	62.9