Hotspot

psbI-trnS-GCU

LSC · 8164-8286 · 123 bp

Back to Maianthemum

Pi 0.0379

MarkerSeek score 72.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 123
variable_sites: 12
pi: 0.0379
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CTTATCTAACGATCCGGGCC	ATTGGGAGAGATGGCTGAGT	55.0	50.0	57.6	58.1	254-2225 bp	1.0	78.9
psbI-trnS-GCU_p2	CTTATCTAACGATCCGGGCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	57.6	58.2	185-2156 bp	1.0	78.5
psbI-trnS-GCU_p3	CTTATCTAACGATCCGGGCC	GTGGACTAAAGCGGCAGATT	55.0	50.0	57.6	58.3	236-2207 bp	1.0	77.8
psbI-trnS-GCU_p4	CTTATCTAACGATCCGGGCC	TGGACTAAAGCGGCAGATTG	55.0	50.0	57.6	58.3	235-2206 bp	1.0	77.8
psbI-trnS-GCU_p5	CTTATCTAACGATCCGGGCC	TTCGAATCCCTCTCTCTCCG	55.0	55.0	57.6	58.3	182-2153 bp	1.0	77.7