Hotspot

ycf1

IRb · 109342-110676 · 1335 bp

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Pi 0.00654

MarkerSeek score 50.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1335
variable_sites: 22
pi: 0.00654
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTTCATAGTGGCTCCGTTCC	TTCCCAGTACCCCCATGAAT	55.0	50.0	58.1	58.0	2619-2700 bp	1.0	80.8
ycf1_p2	TCGTAACTCCCGGAACTTCT	TTCCCAGTACCCCCATGAAT	50.0	50.0	58.1	58.0	2637-2718 bp	1.0	80.6
ycf1_p3	CAATTCCCGTTCTCAACCCA	TTCCCAGTACCCCCATGAAT	50.0	50.0	58.1	58.0	2683-2764 bp	1.0	80.5
ycf1_p4	CTTCATAGTGGCTCCGTTCC	ACTGCAGTCGATCTTCAACC	55.0	50.0	58.1	57.9	1938-2008 bp	1.0	80.4
ycf1_p5	ATTCCCGTTCTCAACCCATG	TTCCCAGTACCCCCATGAAT	50.0	50.0	57.9	58.0	2681-2762 bp	1.0	80.2