Hotspot

ccsA

SSC · 114775-115740 · 966 bp

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Pi 0.00069

MarkerSeek score 32.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 966
variable_sites: 2
pi: 0.00069
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TGGCTCTTTCAATCTCGACG	CGGTTTTGTTGCGGAATTGA	50.0	45.0	58.0	57.9	1760-1761 bp	1.0	79.9
ccsA_p2	GTGAAATCGGTAGACACGCT	CGGTTTTGTTGCGGAATTGA	50.0	45.0	58.0	57.9	1696-1697 bp	1.0	79.9
ccsA_p3	TGAAATCGGTAGACACGCTG	CGGTTTTGTTGCGGAATTGA	50.0	45.0	58.0	57.9	1695-1696 bp	1.0	79.9
ccsA_p4	TGGCTCTTTCAATCTCGACG	CTTCTCTTGCATTGCCAGGA	50.0	50.0	58.0	58.2	1785-1786 bp	1.0	79.6
ccsA_p5	GTGAAATCGGTAGACACGCT	CTTCTCTTGCATTGCCAGGA	50.0	50.0	58.0	58.2	1721-1722 bp	1.0	79.6