Hotspot

accD

LSC · 59269-60765 · 1497 bp

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Pi 0.0117

MarkerSeek score 51.90

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 38
pi: 0.0117
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CGGGATTGGGATTGGGAAAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2483-2668 bp	1.0	84.7
accD_p2	TTCGTTCAAATAGGGGGCAG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2023-2142 bp	1.0	84.5
accD_p3	TGGACCCCTCCAATGTACTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	2582 bp	0.166667	46.0
accD_p4	TGGACCCCTCCAATGTACTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.9	57.9	2588 bp	0.166667	45.4
accD_p5	TGGACCCCTCCAATGTACTT	GCATAACAGCGCTTTCACAG	50.0	50.0	57.9	58.1	2751 bp	0.166667	45.3