Hotspot

ycf15-trnI-CAU

IRa · 145345-151872 · 6528 bp

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Pi 0.000691
MarkerSeek score 54.50
Primer available yes
Label ycf15-trnI-CAU

Feature Metadata

label_name
ycf15-trnI-CAU
feature_type
igs
region
IRa
length_bp
6528
variable_sites
12
pi
0.000691
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnI-CAU_p1 GATTGCTTGTTGAACCCTGC TGGGGATTAGCATATATTTCAAGT 50.0 33.3 57.9 55.7 512-663 bp 1.0 45.4
ycf15-trnI-CAU_p2 TAGGAAGAAGCCCCCTCAAA TGGGGATTAGCATATATTTCAAGT 50.0 33.3 57.9 55.7 626-777 bp 1.0 45.2
ycf15-trnI-CAU_p3 GGTATAGGAAGAAGCCCCCT TGGGGATTAGCATATATTTCAAGT 55.0 33.3 57.9 55.7 630-781 bp 1.0 45.2
ycf15-trnI-CAU_p4 TCTCAGTAGGAGTCGAACCC TGGGGATTAGCATATATTTCAAGT 55.0 33.3 57.9 55.7 908-1059 bp 1.0 44.8
ycf15-trnI-CAU_p5 CAGTAGGAGTCGAACCCAGA TGGGGATTAGCATATATTTCAAGT 55.0 33.3 58.2 55.7 905-1056 bp 1.0 44.8