Hotspot

ndhG-ndhI

SSC · 119236-119707 · 472 bp

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Pi 0.00664

MarkerSeek score 56.50

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 472
variable_sites: 7
pi: 0.00664
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AATCATGTATTGGCGCAGGT	TCAAATTGCTTTGGGTCGGT	45.0	45.0	57.9	58.3	590-605 bp	1.0	79.3
ndhG-ndhI_p2	ATTGGCGCAGGTAAATCCAT	TCAAATTGCTTTGGGTCGGT	45.0	45.0	57.9	58.3	582-597 bp	1.0	79.2
ndhG-ndhI_p3	CGCCGACTATAAGACCCAAC	TCAAATTGCTTTGGGTCGGT	55.0	45.0	58.2	58.3	629-644 bp	1.0	78.4
ndhG-ndhI_p4	ACTCCTAAACCGCCGACTAT	TCAAATTGCTTTGGGTCGGT	50.0	45.0	58.2	58.3	639-654 bp	1.0	77.8
ndhG-ndhI_p5	GCCGACTATAAGACCCAACC	TCAAATTGCTTTGGGTCGGT	55.0	45.0	57.8	58.3	628-643 bp	1.0	77.7