Hotspot

ycf1-ndhF

SSC · 110677-110783 · 107 bp

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Pi 0.0299

MarkerSeek score 57.40

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 107
variable_sites: 7
pi: 0.0299
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GCATCCACGTACAATCCACA	TTCCCAGTACCCCCATGAAT	50.0	50.0	58.3	58.0	875-889 bp	1.0	79.7
ycf1-ndhF_p2	CGAAATTACTCTGCGGTGGA	TTCCCAGTACCCCCATGAAT	50.0	50.0	58.0	58.0	823 bp	0.333333	53.3
ycf1-ndhF_p3	CGAAATTACTCTGCGGTGGA	TTTCCCAGTACCCCCATGAA	50.0	50.0	58.0	58.2	824 bp	0.333333	51.5
ycf1-ndhF_p4	CGAAATTACTCTGCGGTGGA	AAATATATAGGGCGCGGACG	50.0	50.0	58.0	57.7	288 bp	0.333333	51.3
ycf1-ndhF_p5	CGAAATTACTCTGCGGTGGA	AAAAGCTTTCCCAGTACCCC	50.0	50.0	58.0	57.7	830 bp	0.333333	51.0