Hotspot

trnI-CAU-ycf2

IRb · 87420-87749 · 330 bp

Back to Mahonia

Pi 0.0164

MarkerSeek score 67.80

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 330
variable_sites: 12
pi: 0.0164
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	ATAGACGTCGTTTTGCCCTG	50.0	50.0	58.0	58.3	347-498 bp	1.0	84.1
trnI-CAU-ycf2_p2	AGTTGGGCGCTTTAACCATT	ATAGACGTCGTTTTGCCCTG	45.0	50.0	58.4	58.3	348-499 bp	1.0	81.1
trnI-CAU-ycf2_p3	TTGGGCGCTTTAACCATTCA	ATAGACGTCGTTTTGCCCTG	45.0	50.0	58.4	58.3	346-497 bp	1.0	81.1
trnI-CAU-ycf2_p4	CCAATTATGAGTTGGGCGCT	ATAGACGTCGTTTTGCCCTG	50.0	50.0	58.6	58.3	357-508 bp	1.0	79.2
trnI-CAU-ycf2_p5	GAGTTGGGCGCTTTAACCAT	ATAGACGTCGTTTTGCCCTG	50.0	50.0	58.8	58.3	349-500 bp	1.0	77.5