Hotspot

ndhC-trnV-UAC

LSC · 51572-53147 · 1576 bp

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Pi 0.00294
MarkerSeek score 63.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1576
variable_sites
12
pi
0.00294
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGTCCTTCACTAATTGGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1685-1723 bp 1.0 80.9
ndhC-trnV-UAC_p2 CCGGTCCTTCACTAATTGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1686-1724 bp 1.0 80.8
ndhC-trnV-UAC_p3 CCTTCACTAATTGGGGCCAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1681-1719 bp 1.0 79.3
ndhC-trnV-UAC_p4 GGTCCTTCACTAATTGGGGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 1684-1722 bp 1.0 79.3
ndhC-trnV-UAC_p5 GTCCTTCACTAATTGGGGCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 1683-1721 bp 1.0 79.3