Hotspot

petN-psbM

LSC · 28697-29347 · 651 bp

Back to Mahonia

Pi 0.00308
MarkerSeek score 61.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
651
variable_sites
6
pi
0.00308
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCTTGCTTGGGCTGCTTTAA TGCTACTGCATTGTTCATTCT 45.0 38.1 58.0 55.9 804-806 bp 1.0 55.7
petN-psbM_p2 AGTAAGTCTTGCTTGGGCTG TGCTACTGCATTGTTCATTCT 50.0 38.1 57.8 55.9 810-812 bp 1.0 54.2
petN-psbM_p3 ATGGGGAAGAAGTGGACTCT TGCTACTGCATTGTTCATTCT 50.0 38.1 57.6 55.9 750-752 bp 1.0 53.0
petN-psbM_p4 TCTTGCTTGGGCTGCTTTAA TGCTACTGCATTGTTCATTC 45.0 40.0 58.0 54.3 804-806 bp 1.0 51.1
petN-psbM_p5 TTGCTTGGGCTGCTTTAATG TGCTACTGCATTGTTCATTCT 45.0 38.1 57.3 55.9 802-804 bp 1.0 49.8