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trnK-UUU-rps16

LSC · 4419-5009 · 591 bp

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Pi 0.00575

MarkerSeek score 65.70

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 591
variable_sites: 9
pi: 0.00575
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	TCTACCAACGGTATGGACGA	AAAAAGGCGCTCAACCTACA	50.0	45.0	57.9	58.0	746-758 bp	1.0	81.3
trnK-UUU-rps16_p2	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	681-693 bp	1.0	81.3
trnK-UUU-rps16_p3	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.2	58.0	680-692 bp	1.0	81.3
trnK-UUU-rps16_p4	TCTAGCCGCACTTAAAAGCC	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	695-707 bp	1.0	80.4
trnK-UUU-rps16_p5	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	50.0	45.0	58.3	58.0	690-702 bp	1.0	80.3