Hotspot

psaC

SSC · 122229-122474 · 246 bp

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Pi 0.00233
MarkerSeek score 25.80
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
7
pi
0.00233
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 AAGTAGAATTTTGTGAAACGTA GATTCGTCTGGTGTCTACAA 27.3 45.0 52.0 54.9 80-363 bp 1.0 43.0
psaC_p2 TAAGTAGAATTTTGTGAAACGT GATTCGTCTGGTGTCTACAA 27.3 45.0 52.0 54.9 80-364 bp 1.0 43.0
psaC_p3 TTAAGTAGAATTTTGTGAAACGT GATTCGTCTGGTGTCTACAA 26.1 45.0 52.9 54.9 80-365 bp 1.0 43.0
psaC_p4 TTTAAGTAGAATTTTGTGAAACGT GATTCGTCTGGTGTCTACAA 25.0 45.0 53.7 54.9 80-366 bp 1.0 43.0
psaC_p5 AAGTAGAATTTTGTGAAACGTA GATTCGTCTGGTGTCTACAAT 27.3 42.9 52.0 55.4 80-363 bp 1.0 43.0