Hotspot

ndhF

SSC · 114491-116716 · 2226 bp

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Pi 0.00527

MarkerSeek score 44.60

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 186
pi: 0.00527
unique_haplotype_count: 91
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAAGGGGACTAAACAGGA	GAGAAGAGCTGGTGAATCGG	50.0	55.0	58.0	58.1	80-2854 bp	0.915584	86.8
ndhF_p2	ACGAAGGGGACTAAACAGGA	AGAGCTGGTGAATCGGAAAC	50.0	50.0	58.0	57.9	80-2850 bp	0.915584	86.5
ndhF_p3	AGGCACAACTTGAGCAATGA	GGTCGAGTTATTGGCGGATT	45.0	50.0	58.0	58.1	80-2720 bp	0.168831	51.1
ndhF_p4	AGGCACAACTTGAGCAATGA	GAGAAGAGCTGGTGAATCGG	45.0	55.0	58.0	58.1	80-2373 bp	0.168831	50.9
ndhF_p5	AGGCACAACTTGAGCAATGA	AGAGCTGGTGAATCGGAAAC	45.0	50.0	58.0	57.9	80-2369 bp	0.168831	50.6