Hotspot

petA-psbJ

LSC · 66475-67649 · 1175 bp

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Pi 0.0198

MarkerSeek score 67.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1175
variable_sites: 220
pi: 0.0198
unique_haplotype_count: 116
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	45.0	50.0	58.2	57.1	80-1343 bp	1.0	83.5
petA-psbJ_p2	ACGTGTCCAAGGTTTGTTGT	GGTTCATATTCCGGATTGGGA	45.0	47.6	58.2	57.5	80-1350 bp	1.0	78.6
petA-psbJ_p3	TGGCATCTGTTATTCTGGCA	ATTCCGGATTGGGATCATCC	45.0	50.0	57.2	57.1	80-1317 bp	0.987013	77.9
petA-psbJ_p4	ACGTGTCCAAGGTTTGTTGT	CGGTTCATATTCCGGATTGG	45.0	50.0	58.2	56.4	80-1351 bp	1.0	77.6
petA-psbJ_p5	CGTGTCCAAGGTTTGTTGTT	ATTCCGGATTGGGATCATCC	45.0	50.0	57.1	57.1	80-1342 bp	1.0	77.5