Hotspot

ndhC-trnV-UAC

LSC · 52796-54581 · 1786 bp

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Pi 0.00686

MarkerSeek score 70.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1786
variable_sites: 190
pi: 0.00686
unique_haplotype_count: 103
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	80-1970 bp	1.0	89.3
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	80-1969 bp	1.0	86.6
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	80-1983 bp	0.987013	86.2
ndhC-trnV-UAC_p4	CTGGTCCTTCACTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.4	57.8	80-1987 bp	1.0	85.6
ndhC-trnV-UAC_p5	TAATCGGGGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.4	57.8	80-1975 bp	1.0	85.6