Hotspot

ycf3-trnS-GGA

LSC · 46768-47201 · 434 bp

Back to Magnolia

Pi 0.00672
MarkerSeek score 61.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
434
variable_sites
43
pi
0.00672
unique_haplotype_count
52
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 534-660 bp 1.0 82.1
ycf3-trnS-GGA_p2 GCCTCCTTTTCTCCTGAAGT TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 533-659 bp 1.0 82.1
ycf3-trnS-GGA_p3 TGCCTCCTTTTCTCCTGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 584-710 bp 1.0 81.9
ycf3-trnS-GGA_p4 GCCTCCTTTTCTCCTGAAGT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 583-709 bp 1.0 81.9
ycf3-trnS-GGA_p5 GCCTCCTTTTCTCCTGAAGTT ACGGAAAGAGAGGGATTCGA 47.6 50.0 57.9 58.2 583-709 bp 1.0 79.6