Hotspot

atpH-atpI

LSC · 14750-15546 · 797 bp

Back to Magnolia

Pi 0.00684

MarkerSeek score 69.00

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 78
pi: 0.00684
unique_haplotype_count: 85
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	885-1191 bp	1.0	85.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	941-1247 bp	1.0	84.4
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	CAAGCGGGATTCAAGCTCTT	45.0	50.0	58.0	58.5	906-1212 bp	0.993506	84.4
atpH-atpI_p4	GATACCTTCTACGGCTTGGC	TTTTTGCAACTTTAGCCGCG	55.0	45.0	58.1	58.5	970-1276 bp	1.0	84.3
atpH-atpI_p5	CGATACCTTCTACGGCTTGG	TTTTTGCAACTTTAGCCGCG	55.0	45.0	57.9	58.5	971-1277 bp	1.0	84.2