Hotspot

trnS-GCU-trnG-UCC

LSC · 9335-10005 · 671 bp

Back to Magnolia

Pi 0.00602

MarkerSeek score 65.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 69
pi: 0.00602
unique_haplotype_count: 60
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGTCGCTTTAGTCCA	AATCAAACCGAAAGACCCCC	50.0	50.0	58.0	58.1	774-888 bp	1.0	90.3
trnS-GCU-trnG-UCC_p2	AATCCGTCGCTTTAGTCCAC	AATCAAACCGAAAGACCCCC	50.0	50.0	58.0	58.1	773-887 bp	1.0	90.3
trnS-GCU-trnG-UCC_p3	CAATCCGTCGCTTTAGTCCA	AACCGAAAGACCCCCTAACT	50.0	50.0	58.0	58.3	769-883 bp	1.0	88.9
trnS-GCU-trnG-UCC_p4	AATCCGTCGCTTTAGTCCAC	AACCGAAAGACCCCCTAACT	50.0	50.0	58.0	58.3	768-882 bp	1.0	88.9
trnS-GCU-trnG-UCC_p5	CAATCCGTCGCTTTAGTCCA	TCAAACCGAAAGACCCCCTA	50.0	50.0	58.0	58.3	772-886 bp	1.0	88.8