Hotspot

psbK-psbI

LSC · 8595-8951 · 357 bp

Back to Magnolia

Pi 0.00889

MarkerSeek score 60.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 357
variable_sites: 39
pi: 0.00889
unique_haplotype_count: 42
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCCAAGATCATTTTTGGGGGA	50.0	42.9	57.9	57.2	404-431 bp	1.0	76.8
psbK-psbI_p2	GCCAAATTACCCGAGGCTTA	TCCAAGATCATTTTTGGGGGA	50.0	42.9	58.0	57.2	491-518 bp	1.0	76.3
psbK-psbI_p3	ACCCGAGGCTTATGCTTTTT	TCCAAGATCATTTTTGGGGGA	45.0	42.9	57.8	57.2	483-510 bp	1.0	75.0
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	TCCAAGATCATTTTTGGGGGA	45.0	42.9	58.3	57.2	401-428 bp	1.0	74.8
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	TCCAAGATCATTTTTGGGGGA	45.0	42.9	58.3	57.2	402-429 bp	1.0	74.8