Hotspot

rps16-trnQ-UUG

LSC · 6085-8007 · 1923 bp

Back to Magnolia

Pi 0.00782
MarkerSeek score 68.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1923
variable_sites
229
pi
0.00782
unique_haplotype_count
117
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAAGAATCCACAGCCGATCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1225-2092 bp 1.0 91.2
rps16-trnQ-UUG_p2 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1215-2082 bp 1.0 91.2
rps16-trnQ-UUG_p3 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1213-2080 bp 1.0 91.2
rps16-trnQ-UUG_p4 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1221-2088 bp 1.0 91.1
rps16-trnQ-UUG_p5 GAATCCACAGCCGATCATGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1222-2089 bp 1.0 91.1