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trnH-GUG-psbA

LSC · 87-469 · 383 bp

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Pi 0.0162

MarkerSeek score 59.80

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 383
variable_sites: 66
pi: 0.0162
unique_haplotype_count: 89
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	TCACAATCCACTGCCTTGAG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.8	57.9	450-488 bp	1.0	90.0
trnH-GUG-psbA_p2	TCACAATCCACTGCCTTGAG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.8	57.9	453-578 bp	1.0	89.9
trnH-GUG-psbA_p3	TCACAATCCACTGCCTTGAG	AGCTGCTGTTGAAGTTCCAT	50.0	45.0	57.8	57.7	439-477 bp	1.0	88.2
trnH-GUG-psbA_p4	CGCATGGTGGATTCACAATC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.5	57.9	462-500 bp	1.0	87.6
trnH-GUG-psbA_p5	CGCATGGTGGATTCACAATC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.5	57.9	465-590 bp	1.0	87.6