Hotspot

ndhF

SSC · 128839-131079 · 2241 bp

Back to Maesa

Pi 0.00669
MarkerSeek score 47.00
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2241
variable_sites
22
pi
0.00669
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 ACCTGGTGACTCGAAAACAA GCAGAATACCGTCACCCATT 45.0 50.0 57.3 58.0 2589-2615 bp 1.0 75.4
ndhF_p2 ACCTGGTGACTCGAAAACAA ACAGGAACAAGAGGGATCCA 45.0 50.0 57.3 58.0 2486-2512 bp 1.0 75.4
ndhF_p3 ACCTGGTGACTCGAAAACAA AAGAGGGATCCACCGAAGAA 45.0 50.0 57.3 58.0 2478-2504 bp 1.0 75.4
ndhF_p4 ACCTGGTGACTCGAAAACAA AGGGATCCACCGAAGAAGAT 45.0 50.0 57.3 57.8 2475-2501 bp 1.0 74.3
ndhF_p5 ACCTGGTGACTCGAAAACAA CAGGAACAAGAGGGATCCAC 45.0 55.0 57.3 57.6 2485-2511 bp 1.0 72.4