Hotspot

infA-rps8

LSC · 83032-83157 · 126 bp

Pi 0.016

MarkerSeek score 64.30

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 126
variable_sites: 3
pi: 0.016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	CGAACCCGGAACATACCATT	TCGACTAAAAGGAATCGGCG	50.0	50.0	58.0	58.1	242-246 bp	1.0	80.7
infA-rps8_p2	CGAACCCGGAACATACCATT	GGCGGAATGGGAGTTGTAAT	50.0	50.0	58.0	57.9	301-305 bp	1.0	80.6
infA-rps8_p3	CGAACCCGGAACATACCATT	TTTAGGCGGAATGGGAGTTG	50.0	50.0	58.0	57.9	305-309 bp	1.0	80.1
infA-rps8_p4	CGAACCCGGAACATACCATT	TAGGCGGAATGGGAGTTGTA	50.0	50.0	58.0	58.1	303-307 bp	1.0	80.0
infA-rps8_p5	AACCCGGAACATACCATTGG	GGCGGAATGGGAGTTGTAAT	50.0	50.0	57.9	57.9	299-303 bp	1.0	79.8