Hotspot

psaJ-rpl33

LSC · 70160-70618 · 459 bp

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Pi 0.00585
MarkerSeek score 47.60
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
459
variable_sites
4
pi
0.00585
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ATGCGTTGACATTCCCCTTT GGACATCTTTACCCTTGGCC 45.0 55.0 58.1 58.2 511-513 bp 1.0 78.6
psaJ-rpl33_p2 TGCGTTGACATTCCCCTTTT GGACATCTTTACCCTTGGCC 45.0 55.0 58.3 58.2 510-512 bp 1.0 76.8
psaJ-rpl33_p3 ATGCGTTGACATTCCCCTTT GACATCTTTACCCTTGGCCA 45.0 50.0 58.1 57.5 510-512 bp 1.0 76.4
psaJ-rpl33_p4 ATGCGTTGACATTCCCCTTT CGGACATCTTTACCCTTGGC 45.0 55.0 58.1 58.6 512-514 bp 1.0 75.5
psaJ-rpl33_p5 GATGCGTTGACATTCCCCTT GGACATCTTTACCCTTGGCC 50.0 55.0 58.5 58.2 512-514 bp 1.0 74.9