Hotspot

accD-psaI

LSC · 61227-61984 · 758 bp

Back to Maesa

Pi 0.0044
MarkerSeek score 61.60
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
758
variable_sites
5
pi
0.0044
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCCAGAAGGGCTTATTCGATC AAAGAAGCCATTGCCATTGC 47.6 45.0 57.2 57.9 948 bp 1.0 65.6
accD-psaI_p2 CCAGAAGGGCTTATTCGATCT AAAGAAGCCATTGCCATTGC 47.6 45.0 57.2 57.9 947 bp 1.0 65.5
accD-psaI_p3 TTCCAGAAGGGCTTATTCGA AAAGAAGCCATTGCCATTGC 45.0 45.0 56.2 57.9 949 bp 1.0 65.5
accD-psaI_p4 TCCAGAAGGGCTTATTCGATC TTGCCATTGCCGGAAATACT 47.6 45.0 57.2 58.2 938 bp 1.0 65.2
accD-psaI_p5 CCAGAAGGGCTTATTCGATCT TTGCCATTGCCGGAAATACT 47.6 45.0 57.2 58.2 937 bp 1.0 65.1