Hotspot

ndhC-trnV-UAC

LSC · 52727-53870 · 1144 bp

Back to Maesa

Pi 0.00875
MarkerSeek score 56.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1144
variable_sites
15
pi
0.00875
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGCTAATCGGGGCTAAAAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.0 57.8 1284-1333 bp 1.0 79.0
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1275-1324 bp 1.0 78.3
ndhC-trnV-UAC_p3 CTCTGGTCCTTGGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.8 57.8 1294-1343 bp 1.0 77.5
ndhC-trnV-UAC_p4 CTTGGCTAATCGGGGCTAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1286-1335 bp 1.0 76.7
ndhC-trnV-UAC_p5 GGCTAATCGGGGCTAAAACT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1283-1332 bp 1.0 76.6