Hotspot

rps2-rpoC2

LSC · 16961-17180 · 220 bp

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Pi 0.0199
MarkerSeek score 66.70
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
220
variable_sites
6
pi
0.0199
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 AACTCCTGCCTCCATCATCT TCCACGATTCATCAGAACAATCA 50.0 39.1 58.1 58.2 321-332 bp 1.0 56.2
rps2-rpoC2_p2 TGCCTCCATCATCTCTTCCA TCCACGATTCATCAGAACAATCA 50.0 39.1 58.1 58.2 315-326 bp 1.0 56.2
rps2-rpoC2_p3 AACTCCTGCCTCCATCATCT CCACGATTCATCAGAACAATCA 50.0 40.9 58.1 56.8 320-331 bp 1.0 55.9
rps2-rpoC2_p4 TGCCTCCATCATCTCTTCCA CCACGATTCATCAGAACAATCA 50.0 40.9 58.1 56.8 314-325 bp 1.0 55.9
rps2-rpoC2_p5 TCCTGCCTCCATCATCTCTT TCCACGATTCATCAGAACAATCA 50.0 39.1 57.8 58.2 318-329 bp 1.0 55.6