Hotspot

atpH-atpI

LSC · 14232-15292 · 1061 bp

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Pi 0.00633
MarkerSeek score 63.40
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1061
variable_sites
10
pi
0.00633
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT AAGCGGCATTCAAGCTCTTA 45.0 45.0 58.0 57.9 1163-1169 bp 1.0 79.9
atpH-atpI_p2 TACCTTGACCAACTCCAGGT AAGCGGCATTCAAGCTCTTA 50.0 45.0 57.9 57.9 1219-1225 bp 1.0 79.3
atpH-atpI_p3 AATAACGGAAGCGGCAGAAA AAGCGGCATTCAAGCTCTTA 45.0 45.0 58.2 57.9 1164-1170 bp 1.0 78.8
atpH-atpI_p4 GCAGTACCTTGACCAACTCC AAGCGGCATTCAAGCTCTTA 55.0 45.0 58.2 57.9 1223-1229 bp 1.0 78.7
atpH-atpI_p5 GCCAATCCAGCAGCAATAAC AAGCGGCATTCAAGCTCTTA 50.0 45.0 57.8 57.9 1178-1184 bp 1.0 78.5