Hotspot

atpF-atpH

LSC · 13599-13985 · 387 bp

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Pi 0.0103
MarkerSeek score 62.70
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
387
variable_sites
6
pi
0.0103
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG GGACTGGTTGTAGCATTAGCA 50.0 47.6 57.9 58.0 497-509 bp 1.0 73.0
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG TGGACTGGTTGTAGCATTAGC 50.0 47.6 57.9 58.0 498-510 bp 1.0 73.0
atpF-atpH_p3 ATTAAACCCGAAACTCCCGG GCGGAGGGGAAAATACGAG 50.0 57.9 57.9 58.0 563-575 bp 1.0 72.8
atpF-atpH_p4 CCAGTAGCCCAAAGAAACGA GGACTGGTTGTAGCATTAGCA 50.0 47.6 57.8 58.0 470-482 bp 1.0 72.6
atpF-atpH_p5 CCAGTAGCCCAAAGAAACGA GCGGAGGGGAAAATACGAG 50.0 57.9 57.8 58.0 536-548 bp 1.0 72.4