Hotspot

rps16-trnQ-UUG

LSC · 6150-7640 · 1491 bp

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Pi 0.00864
MarkerSeek score 65.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1491
variable_sites
18
pi
0.00864
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 1571-1659 bp 1.0 81.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1554-1642 bp 1.0 80.6
rps16-trnQ-UUG_p3 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1572-1660 bp 1.0 80.0
rps16-trnQ-UUG_p4 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1568-1656 bp 1.0 78.7
rps16-trnQ-UUG_p5 AACGGATCGTGTCCTTCAAG CAATTTAAATGGGGCGTGGC 50.0 50.0 57.9 58.3 1628-1638 bp 0.666667 65.2