Hotspot

trnK-UUU-rps16

LSC · 4188-5035 · 848 bp

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Pi 0.0056
MarkerSeek score 65.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
848
variable_sites
6
pi
0.0056
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATCCCTTGCTTCATCCA AACCTACAGAAACGGTTCGG 50.0 50.0 58.0 57.8 998-1008 bp 1.0 80.8
trnK-UUU-rps16_p2 ACGAATCCCTTGCTTCATCC AACCTACAGAAACGGTTCGG 50.0 50.0 58.0 57.8 999-1009 bp 1.0 80.8
trnK-UUU-rps16_p3 ATGGACGAATCCCTTGCTTC AACCTACAGAAACGGTTCGG 50.0 50.0 58.0 57.8 1003-1013 bp 1.0 80.8
trnK-UUU-rps16_p4 GGACGAATCCCTTGCTTCAT AACCTACAGAAACGGTTCGG 50.0 50.0 58.0 57.8 1001-1011 bp 1.0 80.8
trnK-UUU-rps16_p5 CGAATCCCTTGCTTCATCCA TGCTCAACCTACAGAAACGG 50.0 50.0 58.0 57.8 1003-1013 bp 1.0 80.7