Hotspot

ycf1

SSC · 124999-130422 · 5424 bp

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Pi 0.0223
MarkerSeek score 52.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5424
variable_sites
121
pi
0.0223
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGTTGTGTAGTGATTCTTGGA ACAAACCAAGTGATGATTCT 38.1 35.0 54.5 52.7 178 bp 0.5 20.0
ycf1_p2 AGTTGTGTAGTGATTCTTGGA AACAAACCAAGTGATGATTCT 38.1 33.3 54.5 53.6 179 bp 0.5 20.0
ycf1_p3 AAGTTGTGTAGTGATTCTTGGA ACAAACCAAGTGATGATTCT 36.4 35.0 55.3 52.7 179 bp 0.5 20.0
ycf1_p4 AGTTGTGTAGTGATTCTTGGAA ACAAACCAAGTGATGATTCT 36.4 35.0 55.3 52.7 178 bp 0.5 20.0
ycf1_p5 AGTTGTGTAGTGATTCTTGGAA AACAAACCAAGTGATGATTCT 36.4 33.3 55.3 53.6 179 bp 0.5 20.0