Hotspot

rpl22

LSC · 83878-84360 · 483 bp

Pi 0.0352

MarkerSeek score 63.40

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 483
variable_sites: 17
pi: 0.0352
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGTCGATACGTCCTGCAATT	GTGTCCACGGTTATACCCAC	50.0	55.0	58.4	58.0	1197 bp	1.0	78.3
rpl22_p2	TTCGACTCTTCGGGGCTTAT	GTGTCCACGGTTATACCCAC	50.0	55.0	58.5	58.0	968 bp	1.0	78.1
rpl22_p3	CGTCGATACGTCCTGCAATT	ATAAACTTCCGGGGACATGC	50.0	50.0	58.4	57.9	1074 bp	1.0	77.8
rpl22_p4	CGTCGATACGTCCTGCAATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.4	58.1	1387 bp	1.0	77.4
rpl22_p5	CGTCGATACGTCCTGCAATT	GTCGGACAAGTGGGAAATGT	50.0	50.0	58.4	58.1	1618 bp	1.0	77.1