Hotspot

trnL-UAG-ccsA

SSC · 115415-115535 · 121 bp

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Pi 0.0526
MarkerSeek score 76.30
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
121
variable_sites
6
pi
0.0526
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 TAGACACGCTGCTCTTAGGA CAATTGAAACGACCGAAAAGGA 50.0 40.9 57.9 57.8 236-243 bp 1.0 65.6
trnL-UAG-ccsA_p2 GTTTTGAACAAGTTGCCGCT CAATTGAAACGACCGAAAAGGA 45.0 40.9 58.1 57.8 269-276 bp 1.0 65.4
trnL-UAG-ccsA_p3 AAGTTGCCGCTATGGTGAAA CAATTGAAACGACCGAAAAGGA 45.0 40.9 58.1 57.8 260-267 bp 1.0 65.4
trnL-UAG-ccsA_p4 AGTTGCCGCTATGGTGAAAT CAATTGAAACGACCGAAAAGGA 45.0 40.9 57.9 57.8 259-266 bp 1.0 65.2
trnL-UAG-ccsA_p5 AAATTGGTAGACACGCTGCT CAATTGAAACGACCGAAAAGGA 45.0 40.9 57.8 57.8 243-250 bp 1.0 65.0