Hotspot

rpl32-trnL-UAG

SSC · 114219-115334 · 1116 bp

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Pi 0.0529
MarkerSeek score 80.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1116
variable_sites
58
pi
0.0529
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGATATTGGGCAGCATTGA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1261-1268 bp 1.0 84.0
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCATTG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.0 57.9 1262-1269 bp 1.0 84.0
rpl32-trnL-UAG_p3 GGGATATTGGGCAGCATTGA AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 1254-1261 bp 1.0 83.5
rpl32-trnL-UAG_p4 AGGGATATTGGGCAGCATTG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.0 57.8 1255-1262 bp 1.0 83.5
rpl32-trnL-UAG_p5 GGGATATTGGGCAGCATTGA CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.0 57.8 1259-1266 bp 1.0 83.4