Hotspot

infA-rps8

LSC · 80383-80509 · 127 bp

Back to Maerua

Pi 0.075
MarkerSeek score 74.80
Primer available yes
Label infA-rps8

Feature Metadata

label_name
infA-rps8
feature_type
igs
region
LSC
length_bp
127
variable_sites
9
pi
0.075
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA-rps8_p1 ATCCTTTCGGAAGCATGACC TCTCAACGAATTCCGCGAAT 50.0 45.0 58.0 58.0 350-357 bp 1.0 84.6
infA-rps8_p2 CGATCCTTTCGGAAGCATGA TCTCAACGAATTCCGCGAAT 50.0 45.0 58.1 58.0 352-359 bp 1.0 84.4
infA-rps8_p3 TGCCTTCGGAAGTGATTCAG TCTCAACGAATTCCGCGAAT 50.0 45.0 57.9 58.0 288-295 bp 1.0 84.4
infA-rps8_p4 ATCCTTTCGGAAGCATGACC CATCGACTAACATCACCGCA 50.0 50.0 58.0 58.1 554 bp 0.5 59.0
infA-rps8_p5 CGATCCTTTCGGAAGCATGA CATCGACTAACATCACCGCA 50.0 50.0 58.1 58.1 556 bp 0.5 58.9