Hotspot

trnL-UAA-trnF-GAA

LSC · 48536-48894 · 359 bp

Back to Maerua

Pi 0.0288
MarkerSeek score 65.20
Primer available yes
Label trnL-UAA-trnF-GAA

Feature Metadata

label_name
trnL-UAA-trnF-GAA
feature_type
igs
region
LSC
length_bp
359
variable_sites
10
pi
0.0288
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnF-GAA_p1 AATCGTGAGGGTTCAAGTCC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 448-449 bp 1.0 79.7
trnL-UAA-trnF-GAA_p2 AATCGTGAGGGTTCAAGTCC TACCAACTGAGCTATCCCGA 50.0 50.0 57.5 57.6 410-411 bp 1.0 76.9
trnL-UAA-trnF-GAA_p3 AATCGTGAGGGTTCAAGTCC GAACTGGTGACACGAGGATT 50.0 50.0 57.5 57.5 445-446 bp 1.0 76.5
trnL-UAA-trnF-GAA_p4 TTCAAGTCCCTCTATCCCCA TTTGAACTGGTGACACGAGG 50.0 50.0 57.0 58.1 437-438 bp 1.0 75.9
trnL-UAA-trnF-GAA_p5 AATCGTGAGGGTTCAAGTCC TCCTCTGCTCTACCAACTGA 50.0 50.0 57.5 57.4 420-421 bp 1.0 75.6