Hotspot

psbM-trnD-GUC

LSC · 29710-30729 · 1020 bp

Back to Maerua

Pi 0.0278
MarkerSeek score 62.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1020
variable_sites
14
pi
0.0278
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.49
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGAACGAGAATGAAGAGTGC TTCAATTGGTCAGAGCACCG 47.6 50.0 58.0 58.5 624-1138 bp 1.0 73.2
psbM-trnD-GUC_p2 ACGAGAATGAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 45.0 50.0 56.9 58.5 620-1134 bp 1.0 72.5
psbM-trnD-GUC_p3 GGAACGAGAATGAAGAGTGCA TTCAATTGGTCAGAGCACCG 47.6 50.0 58.3 58.5 623-1137 bp 1.0 71.0
psbM-trnD-GUC_p4 AACGAGAATGAAGAGTGCAGT TTCAATTGGTCAGAGCACCG 42.9 50.0 57.6 58.5 621-1135 bp 1.0 70.3
psbM-trnD-GUC_p5 GGAACGAGAATGAAGAGTGC TTCAATTGGTCAGAGCACCG 50.0 50.0 56.5 58.5 623-1137 bp 1.0 69.8